Biotechnologie et Génétique Ingénierie
Plante Génétique Ingénierie - Elements
Description
Recombinant DNUn Technologie or Génétique Ingénierie (GE) has enabled rapid progress en le understanding de genes as also applications de such knowledge pour human benefit. Thus, it is possible now to transfer genes to a species from a wider gene pool covering genes from other species et phyla too. Probably le widest application has been en crop improvement by Plante Génétique Ingénierie, le focus de this book. Le objective de this book is to acquaint le students avec le basic aspects, pour their further explorations into le avenues de this fascinating science et technology. Le book starts avec a brief description de developments en biotechnology, from le art de fermentation to biofermentation, from landrace selection to GM crops. This is followed by descriptions de le basic aspects de gene structure, expression et regulation, et le experimental path, le techniques. Le techniques, covered en several chapters, focus mainly sur Recombinant DNUn construction, gene amplification by cloning or PCR, le principles de plasmid et phage vector designing, construction de genomic et cDNUn libraries et their screening. With this background, global initiatives en applications de these techniques en genome sequencing avec focus sur le Human Genome Project, production de Transgenic GM crops by nuclear transformation et more biosafe Transplastomic GM crops through chloroplast transformation, have also been described. Equal importance has been given to le two steps de GM crop production, formation de le low yielding primary transformant through GE et tissue culture et production de le final high yielding GM crop, by transfer de le transgene from le primary transformant to a selected high yielding variety by conventional plant breeding. Le two steps make le lab to land transfer de transgenic plants possible. All through, le scientist’s concern pour biosafety de GM crops has been highlighted, including le endeavours pour development de Cisgenic plants. Le necessity de coupling advances en crop improvement avec le holistic watershed development approach has also been mentioned. Important aspects de Intellectual Property Rights, Biosafety measures de risk assessment, prevention et mitigation et Biosafety regulations have also been discussed. Le last chapter gives an account de le milestones en le history de molecular biology et recombinant DNUn science avec related references et web links. Le book is intended pour UG et PG students de botany, bioscience, microbiology, et courses related to biotechnology.
Table des matières
Preface, Acknowledgements, Introduction: Overview de Développements en Biotechnologie et Génétique] Modification; Basic Aspects de Moléculaire Biologie; Basic Techniques de Recombinant DNUn Science; Vectors pour Prokaryotic et Eukaryotic Cells; DNUn Libraries et Screening; Genome Components et Sequencing; Production de Transgenic Plantees; Transplastomic Plante Production by Chloroplast Transformation; Intellectual Property Rights et Biosafety Regulations; Timeline de Major Discoveries en Cell et Moléculaire Biologie: Index